Course disputée sur le circuit de Automotodrom Brno
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 99 | Jorge Lorenzo | Fiat Yamaha Team | 03:57:18 | 22 | 25 | 3 |
| 2 | 26 | Dani Pedrosa | Repsol Honda Team | 05:28:52 | 22 | 20 | 1 |
| 3 | 27 | Casey Stoner | Ducati Marlboro Team | 07:07:44 | 22 | 16 | 4 |
| 4 | 11 | Ben Spies | Tech3 Yamaha | 07:46:01 | 22 | 13 | 2 |
| 5 | 46 | Valentino Rossi | Fiat Yamaha Team | 08:56:08 | 22 | 11 | 5 |
| 6 | 69 | Nicky Hayden | Ducati Marlboro Team | 11:24:13 | 22 | 10 | 8 |
| 7 | 5 | Colin Edwards | Tech3 Yamaha | 13:13:54 | 22 | 9 | 7 |
| 8 | 33 | Marco Melandri | San Carlo Honda Gresini | 14:54:04 | 22 | 8 | 14 |
| 9 | 40 | Hector Barbera | Paginas Amarillas Aspar | 14:57:57 | 22 | 7 | 9 |
| 10 | 14 | Randy De Puniet | LCR Honda MotoGP | 15:18:51 | 22 | 6 | 11 |
| 11 | 58 | Marco Simoncelli | San Carlo Honda Gresini | 15:37:50 | 22 | 5 | 12 |
| 12 | 41 | Aleix Espargaro | Pramac Racing | 17:02:09 | 22 | 4 | 16 |
| 13 | 15 | Alex De Angelis | Interwetten Honda MotoGP | 18:13:26 | 22 | 3 | 15 |
| 14 | 19 | Alvaro Bautista | Rizla Suzuki MotoGP | 06:15:19 | 21 | 0 | 17 |
| 15 | 36 | Mika Kallio | Pramac Racing | 20:03:09 | 7 | 0 | 13 |
| 16 | 4 | Andrea Dovizioso | Repsol Honda Team | 01:32:13 | 6 | 0 | 6 |
| 17 | 65 | Loris Capirossi | Rizla Suzuki MotoGP | 12:39:20 | 1 | 0 | 10 |