Course disputée sur le circuit de Assen
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 11 | Ben Spies | Yamaha Factory Racing | 41'44.659 | 26 | 25 | 2 |
| 2 | 27 | Casey Stoner | Repsol Honda Team | 41'52.356 | 26 | 20 | 3 |
| 3 | 4 | Andrea Dovizioso | Repsol Honda Team | 42'12.165 | 26 | 16 | 5 |
| 4 | 46 | Valentino Rossi | Ducati Team | 42'15.343 | 26 | 13 | 11 |
| 5 | 69 | Nicky Hayden | Ducati Team | 42'27.831 | 26 | 11 | 9 |
| 6 | 1 | Jorge Lorenzo | Yamaha Factory Racing | 42'29.195 | 26 | 10 | 4 |
| 7 | 5 | Colin Edwards | Monster Yamaha Tech 3 | 42'52.771 | 26 | 9 | 8 |
| 8 | 7 | Hiroshi Aoyama | San Carlo Honda Gresini | 42'55.412 | 26 | 8 | 12 |
| 9 | 58 | Marco Simoncelli | San Carlo Honda Gresini | 43'09.584 | 26 | 7 | 1 |
| 10 | 24 | Toni Elias | LCR Honda MotoGP | 43'10.875 | 26 | 6 | 15 |
| 11 | 19 | Alvaro Bautista | Rizla Suzuki MotoGP | 43'23.125 | 26 | 5 | 14 |
| 12 | 8 | Hector Barbera | Mapfre Aspar Team MotoGP | 42'11.017 | 25 | 4 | 13 |
| 13 | 64 | Kousuke Akiyoshi | San Carlo Honda Gresini | 42'58.437 | 25 | 3 | 16 |
| 14 | 35 | Cal Crutchlow | Monster Yamaha Tech 3 | 43'07.755 | 24 | 2 | 6 |
| 15 | 14 | Randy De Puniet | Pramac Racing Team | 1'52.494 | 1 | 0 | 10 |
| 16 | 17 | Karel Abraham | Cardion AB Motoracing | 0 | 0 | 7 |