Course disputée sur le circuit de Mugello
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 75 | Mattia Pasini | Team Toth Aprilia | 45'38.391 | 21 | 25 | 8 |
| 2 | 58 | Marco Simoncelli | Metis Gilera | 45'38.508 | 21 | 20 | 2 |
| 3 | 19 | Alvaro Bautista | Mapfre Aspar Team | 45'39.684 | 21 | 16 | 1 |
| 4 | 12 | Thomas Luthi | Emmi - Caffe Latte | 46'02.948 | 21 | 13 | 6 |
| 5 | 40 | Hector Barbera | Pepe World Team | 46'05.405 | 21 | 11 | 3 |
| 6 | 4 | Hiroshi Aoyama | Scot Racing Team 250cc | 46'08.428 | 21 | 10 | 4 |
| 7 | 6 | Alex Debon | Aeropuerto-Castello-Blusens | 46'09.716 | 21 | 9 | 5 |
| 8 | 55 | Hector Faubel | Valencia CF - Honda SAG | 46'13.569 | 21 | 8 | 13 |
| 9 | 35 | Raffaele De Rosa | Scot Racing Team 250cc | 46'23.247 | 21 | 7 | 9 |
| 10 | 15 | Roberto Locatelli | Metis Gilera | 46'24.874 | 21 | 6 | 10 |
| 11 | 25 | Alex Baldolini | WTR San Marino Team | 46'27.912 | 21 | 5 | 17 |
| 12 | 63 | Mike Di Meglio | Mapfre Aspar Team | 46'38.930 | 21 | 4 | 14 |
| 13 | 17 | Karel Abraham | Cardion AB Motoracing | 47'02.121 | 21 | 3 | 11 |
| 14 | 14 | Ratthapark Wilairot | Thai Honda PTT SAG | 47'03.910 | 21 | 2 | 7 |
| 15 | 10 | Imre Toth | Team Toth Aprilia | 47'08.788 | 21 | 1 | 18 |
| 16 | 11 | Balazs Nemeth | Balatonring Team | 47'07.283 | 20 | 0 | 22 |
| 17 | 7 | Alex Pons | Pepe World Team | 47'07.373 | 20 | 0 | 19 |
| 18 | 8 | Bastien Chesaux | Racing Team Germany | 47'07.608 | 20 | 0 | 24 |
| 19 | 53 | Valentin Debise | CIP Moto - GP250 | 47'32.464 | 20 | 0 | 20 |
| 20 | 37 | Daniel Arcas | Milar - Juegos Lucky | 46'39.370 | 18 | 0 | 23 |
| 21 | 48 | Shoya Tomizawa | CIP Moto - GP250 | 13'48.480 | 6 | 0 | 16 |
| 22 | 52 | Lukas Pesek | Auto Kelly - CP | 11'22.134 | 5 | 0 | 15 |
| 23 | 56 | Vladimir Leonov | Viessmann Kiefer Racing | 9'33.535 | 4 | 0 | 21 |
| 24 | 16 | Jules Cluzel | Matteoni Racing | 0 | 0 | 12 |