Course disputée sur le circuit de Mugello
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 75 | Mattia Pasini | Team Toth Aprilia | 17:39:51 | 21 | 25 | 8 |
| 2 | 58 | Marco Simoncelli | Metis Gilera | 17:41:48 | 21 | 20 | 2 |
| 3 | 19 | Alvaro Bautista | Mapfre Aspar Team | 18:01:24 | 21 | 16 | 1 |
| 4 | 12 | Thomas Luthi | Emmi - Caffe Latte | 00:29:08 | 21 | 13 | 6 |
| 5 | 40 | Hector Barbera | Pepe World Team | 01:10:05 | 21 | 11 | 3 |
| 6 | 4 | Hiroshi Aoyama | Scot Racing Team 250cc | 02:00:28 | 21 | 10 | 4 |
| 7 | 6 | Alex Debon | Aeropuerto-Castello-Blusens | 02:21:56 | 21 | 9 | 5 |
| 8 | 55 | Hector Faubel | Valencia CF - Honda SAG | 03:26:09 | 21 | 8 | 13 |
| 9 | 35 | Raffaele De Rosa | Scot Racing Team 250cc | 06:07:27 | 21 | 7 | 9 |
| 10 | 15 | Roberto Locatelli | Metis Gilera | 06:34:34 | 21 | 6 | 10 |
| 11 | 25 | Alex Baldolini | WTR San Marino Team | 07:25:12 | 21 | 5 | 17 |
| 12 | 63 | Mike Di Meglio | Mapfre Aspar Team | 10:28:50 | 21 | 4 | 14 |
| 13 | 17 | Karel Abraham | Cardion AB Motoracing | 16:55:21 | 21 | 3 | 11 |
| 14 | 14 | Ratthapark Wilairot | Thai Honda PTT SAG | 17:25:10 | 21 | 2 | 7 |
| 15 | 10 | Imre Toth | Team Toth Aprilia | 18:46:28 | 21 | 1 | 18 |
| 16 | 11 | Balazs Nemeth | Balatonring Team | 18:21:23 | 20 | 0 | 22 |
| 17 | 7 | Alex Pons | Pepe World Team | 18:22:53 | 20 | 0 | 19 |
| 18 | 8 | Bastien Chesaux | Racing Team Germany | 18:26:48 | 20 | 0 | 24 |
| 19 | 53 | Valentin Debise | CIP Moto - GP250 | 01:21:04 | 20 | 0 | 20 |
| 20 | 37 | Daniel Arcas | Milar - Juegos Lucky | 10:36:10 | 18 | 0 | 23 |
| 21 | 48 | Shoya Tomizawa | CIP Moto - GP250 | 15:08:00 | 6 | 0 | 16 |
| 22 | 52 | Lukas Pesek | Auto Kelly - CP | 22:28:54 | 5 | 0 | 15 |
| 23 | 56 | Vladimir Leonov | Viessmann Kiefer Racing | 16:18:55 | 4 | 0 | 21 |
| 24 | 16 | Jules Cluzel | Matteoni Racing | 01:00:00 | 0 | 0 | 12 |