Course disputée sur le circuit de Automotodrom Brno
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 58 | Marco Simoncelli | Metis Gilera | 41'06.490 | 20 | 25 | 1 |
| 2 | 75 | Mattia Pasini | Team Toth Aprilia | 41'07.174 | 20 | 20 | 7 |
| 3 | 19 | Alvaro Bautista | Mapfre Aspar Team | 41'10.871 | 20 | 16 | 8 |
| 4 | 4 | Hiroshi Aoyama | Scot Racing Team 250cc | 41'15.236 | 20 | 13 | 2 |
| 5 | 15 | Roberto Locatelli | Metis Gilera | 41'16.211 | 20 | 11 | 6 |
| 6 | 35 | Raffaele De Rosa | Scot Racing Team 250cc | 41'17.360 | 20 | 10 | 9 |
| 7 | 40 | Hector Barbera | Pepe World Team | 41'22.129 | 20 | 9 | 3 |
| 8 | 16 | Jules Cluzel | Matteoni Racing | 41'24.901 | 20 | 8 | 14 |
| 9 | 63 | Mike Di Meglio | Mapfre Aspar Team | 41'25.053 | 20 | 7 | 5 |
| 10 | 55 | Hector Faubel | Valencia CF - Honda SAG | 41'26.448 | 20 | 6 | 11 |
| 11 | 14 | Ratthapark Wilairot | Thai Honda PTT SAG | 41'26.574 | 20 | 5 | 12 |
| 12 | 52 | Lukas Pesek | Auto Kelly - CP | 41'37.122 | 20 | 4 | 15 |
| 13 | 48 | Shoya Tomizawa | CIP Moto - GP250 | 41'43.608 | 20 | 3 | 16 |
| 14 | 11 | Balazs Nemeth | Balatonring Team | 42'23.974 | 20 | 2 | 18 |
| 15 | 53 | Valentin Debise | CIP Moto - GP250 | 42'31.174 | 20 | 1 | 20 |
| 16 | 7 | Alex Pons | Pepe World Team | 42'31.622 | 20 | 0 | 19 |
| 17 | 10 | Imre Toth | Team Toth Aprilia | 42'53.415 | 20 | 0 | 22 |
| 18 | 77 | Aitor Rodriguez | Milar - Juegos Lucky | 42'58.955 | 20 | 0 | 24 |
| 19 | 56 | Vladimir Leonov | Viessmann Kiefer Racing | 43'06.284 | 20 | 0 | 23 |
| 20 | 8 | Bastien Chesaux | Racing Team Germany | 25'36.289 | 12 | 0 | 21 |
| 21 | 6 | Alex Debon | Aeropuerto-Castello-Blusens | 19'02.327 | 9 | 0 | 4 |
| 22 | 25 | Alex Baldolini | WTR San Marino Team | 13'54.614 | 6 | 0 | 17 |
| 23 | 54 | Toby Markham | C&L Racing | 2'19.072 | 1 | 0 | 25 |
| 24 | 17 | Karel Abraham | Cardion AB Motoracing | 0 | 0 | 10 | |
| 25 | 12 | Thomas Luthi | Emmi - Caffe Latte | 0 | 0 | 13 |