Course disputée sur le circuit de Mugello
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 1 | Jorge Lorenzo | Yamaha Factory Racing | 02:14:49 | 23 | 25 | 5 |
| 2 | 4 | Andrea Dovizioso | Repsol Honda Team | 02:31:26 | 23 | 20 | 4 |
| 3 | 27 | Casey Stoner | Repsol Honda Team | 02:33:52 | 23 | 16 | 1 |
| 4 | 11 | Ben Spies | Yamaha Factory Racing | 04:44:29 | 23 | 13 | 2 |
| 5 | 58 | Marco Simoncelli | San Carlo Honda Gresini | 04:46:05 | 23 | 11 | 3 |
| 6 | 46 | Valentino Rossi | Ducati Team | 09:35:39 | 23 | 10 | 12 |
| 7 | 8 | Hector Barbera | Mapfre Aspar Team MotoGP | 10:13:54 | 23 | 9 | 10 |
| 8 | 26 | Dani Pedrosa | Repsol Honda Team | 11:08:52 | 23 | 8 | 8 |
| 9 | 5 | Colin Edwards | Monster Yamaha Tech 3 | 11:31:50 | 23 | 7 | 6 |
| 10 | 69 | Nicky Hayden | Ducati Team | 11:53:33 | 23 | 6 | 9 |
| 11 | 7 | Hiroshi Aoyama | San Carlo Honda Gresini | 12:37:28 | 23 | 5 | 13 |
| 12 | 17 | Karel Abraham | Cardion AB Motoracing | 14:27:33 | 23 | 4 | 11 |
| 13 | 19 | Alvaro Bautista | Rizla Suzuki MotoGP | 15:29:03 | 23 | 3 | 14 |
| 14 | 14 | Randy De Puniet | Pramac Racing Team | 15:48:49 | 23 | 2 | 15 |
| 15 | 24 | Toni Elias | LCR Honda MotoGP | 23:08:08 | 23 | 1 | 16 |
| 16 | 35 | Cal Crutchlow | Monster Yamaha Tech 3 | 19:10:47 | 6 | 0 | 7 |