Course disputée sur le circuit de Mugello
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 46 | Valentino Rossi | Fiat Yamaha Team | 13:39:13 | 23 | 25 | 1 |
| 2 | 1 | Casey Stoner | Ducati Marlboro Team | 14:15:54 | 23 | 20 | 4 |
| 3 | 2 | Dani Pedrosa | Repsol Honda Team | 15:00:20 | 23 | 16 | 2 |
| 4 | 15 | Alex De Angelis | San Carlo Honda Gresini | 15:24:26 | 23 | 13 | 10 |
| 5 | 5 | Colin Edwards | Tech3 Yamaha | 17:08:03 | 23 | 11 | 5 |
| 6 | 52 | James Toseland | Tech3 Yamaha | 17:29:19 | 23 | 10 | 8 |
| 7 | 65 | Loris Capirossi | Rizla Suzuki MotoGP | 17:40:00 | 23 | 9 | 3 |
| 8 | 4 | Andrea Dovizioso | Jir Team Scot MotoGP | 17:54:40 | 23 | 8 | 13 |
| 9 | 56 | Shinya Nakano | San Carlo Honda Gresini | 17:54:40 | 23 | 7 | 9 |
| 10 | 7 | Chris Vermeulen | Rizla Suzuki MotoGP | 22:12:18 | 23 | 6 | 11 |
| 11 | 50 | Sylvain Guintoli | Alice Team | 00:39:34 | 23 | 5 | 17 |
| 12 | 24 | Toni Elias | Alice Team | 03:32:54 | 23 | 4 | 16 |
| 13 | 69 | Nicky Hayden | Repsol Honda Team | 03:39:53 | 23 | 3 | 6 |
| 14 | 8 | Tadayuki Okada | Rizla Suzuki MotoGP | 06:00:02 | 23 | 2 | 15 |
| 15 | 13 | Anthony West | Kawasaki Racing Team | 06:31:29 | 23 | 1 | 19 |
| 16 | 21 | John Hopkins | Kawasaki Racing Team | 21:13:49 | 17 | 0 | 14 |
| 17 | 48 | Jorge Lorenzo | Fiat Yamaha Team | 19:31:29 | 17 | 0 | 7 |
| 18 | 33 | Marco Melandri | Ducati Marlboro Team | 14:19:18 | 18 | 0 | 18 |
| 19 | 14 | Randy De Puniet | LCR Honda MotoGP | 14:13:09 | 18 | 0 | 12 |