Course disputée sur le circuit de Automotodrom Brno
| Position | Numéro | Pilote | Team | Temps | Tours | Points | Départ |
|---|---|---|---|---|---|---|---|
| 1 | 46 | Valentino Rossi | Fiat Yamaha Team | 43'08.991 | 22 | 25 | 1 |
| 2 | 3 | Dani Pedrosa | Repsol Honda Team | 43'20.757 | 22 | 20 | 3 |
| 3 | 24 | Toni Elias | San Carlo Honda Gresini | 43'29.747 | 22 | 16 | 4 |
| 4 | 4 | Andrea Dovizioso | Repsol Honda Team | 43'30.409 | 22 | 13 | 6 |
| 5 | 65 | Loris Capirossi | Rizla Suzuki MotoGP | 43'30.529 | 22 | 11 | 9 |
| 6 | 69 | Nicky Hayden | Ducati Marlboro Team | 43'34.535 | 22 | 10 | 8 |
| 7 | 5 | Colin Edwards | Tech3 Yamaha | 43'34.667 | 22 | 9 | 5 |
| 8 | 15 | Alex De Angelis | San Carlo Honda Gresini | 43'43.100 | 22 | 8 | 7 |
| 9 | 52 | James Toseland | Tech3 Yamaha | 43'44.608 | 22 | 7 | 14 |
| 10 | 14 | Randy De Puniet | LCR Honda MotoGP | 43'48.815 | 22 | 6 | 13 |
| 11 | 7 | Chris Vermeulen | Rizla Suzuki MotoGP | 43'49.767 | 22 | 5 | 11 |
| 12 | 88 | Niccolo Canepa | Pramac Racing | 43'59.652 | 22 | 4 | 12 |
| 13 | 41 | Gabor Talmacsi | Scot Racing Team MotoGP | 44'08.179 | 22 | 3 | 17 |
| 14 | 33 | Marco Melandri | Kawasaki Racing Team | 39'38.518 | 20 | 0 | 15 |
| 15 | 36 | Mika Kallio | Pramac Racing | 39'38.278 | 20 | 0 | 10 |
| 16 | 99 | Jorge Lorenzo | Fiat Yamaha Team | 33'15.284 | 17 | 0 | 2 |
| 17 | 84 | Michel Fabrizio | Pramac d\'Antin | 12'13.902 | 6 | 0 | 16 |